![]() Antibodies specifically bind unique pathogen molecules called antigens.Īntibodies exist as one or more copies of a Y-shaped unit composed of four polypeptide chains (Fig. coli system, please specify in your request.Guide to the structural components that make up an antibody - heavy chains, light chains, F(ab)/Fc regions - and antibody isotypes.Īntibodies, also known as immunoglobulins (Ig), are large, Y-shaped glycoproteins produced by B-cells as a primary immune defense. coli expression system as well, if you prefer E. We provide Fab antibody production in E.HEK293 or CHO cell (please specify, cost for production in CHO cell is higher).However, for some risky antibody production projects, for example, production of antibodies with modified constant regions, we would need to charge a small amount of cost for feasibility pilot study and process development (if needed).Ĭonstruction and production details about our Fab fragment antibody service Host cells For antibody production request, we usually quote our price based on successful completion of the production project, and you only need to pay upon receiving the purified antibodies and invoice. ![]() A protease cleavage site can be designed between the His-tag and the Fab to allow tag-removal after purification if needed.įor customers that have set up a contract service account with us, we typically do not require an upfront payment for antibody production orders. coli expression system for Fab antibody production.īecause many antibody Fab fragment proteins do not bind well to protein A, protein G, or protein L affinity resin, we typically add a poly-histidine tag at the C-terminus of the antibody heave chain for purification. If customers prefer, we can also use our E. The recombinant antibody Fab fragment protein produced by our transient transfection in mammalian cells is of superior quality than antibody fragment protein generated from enzyme cleavage of full antibodies. Sino Biological has developed transient transfection technology in HEK293 cells for Fab fragment antibody expression and production, and has generated excellent quality of Fab fragment for antigen-Fab co-crystallization. Besides, like scFv antibodies, it could be easier to generate Fab antibodies with higher affinities. Firstly, it can rapidly and efficiently select new antibodies that are difficult to gain through hybridoma technology. Fab antibody library has many advantages. In contrast to some of the heavily engineered antibody fragments, the Fab has native sequence subsequently, it is less likely for the Fab to be immunogenic when used as therapy.ĭue to these features, the production of antibody fragments is industrially highly demanded and the antibody fragment pipeline is expanding, with three therapeutic Fabs approved by FDA, and many in the active clinical pipeline and preclinical research.įab antibody fragments can be produced by phage display antibody library. The Fab fragment has 440-450 amino acids and its molecular weight is approximately 55 kDa.Īntibody fragments (Fabs), lacking the glycosylated Fc constant regions, have the advantage over whole antibodies in applications requiring rapid tissue penetration and rapid clearance from the blood or kidney. The Fab can be further divided into a variable fragment (Fv) composed of the V H and V L domains, and a constant fragment (Fb) composed of the C L and C H1 domains. It contains one complete L chain in its entirety and the V and C H1 portion of one H chain.
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